RAC1 antibody

Catalog No. ABIN6654500

This anti-RAC1 antibody is a Mouse Monoclonal antibody detecting RAC1 in WB and IHC (p). Suitable for Human.

Quick Overview for RAC1 antibody (ABIN6654500)

Target: RAC1

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CPTC-RAC1-1

Product Details anti-RAC1 Antibody

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: Full length recombinant human protein was used as the immunogen for this RAC1 antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the RAC1 antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the RAC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for RAC1

Target: RAC1

Background: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Rac1 has been demonstrated to play a critical role in tumor progression of human colorectal adenocarcinoma cells. In one study, overexpression of Rac1 accelerated the tumorigenic process, whereas Rac1 inhibition completed suppressed tumor formation.

Pathways: WNT Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling